custom-designed oligonucleotide-based dna microarray  (Lallemand inc)

Journal: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America

Article Title: Fecal Microbial Transplants Reduce Antibiotic-resistant Genes in Patients With Recurrent Clostridium difficile Infection

doi: 10.1093/cid/ciw185

Figure Lengend Snippet: Pearson similarity index of donors over time and recurrent Clostridium difficile infection (RCDI) patients to donors. Samples were analyzed using a DNA microarray. A , Donors retained a high degree of similarity to their own samples over their donation time period. Donor 1 = 18 months; Donor 2 = 12 months; Donor 3 = 7 months ( B ) Percent similarity of the single fecal microbiota transplantation (FMT) recipient samples at those time points (1 = 1–4 weeks; 2 = 8–52 weeks post FMT [PFT]) to their respective donor. Following FMT, the similarity of the RCDI patients increased to more closely resemble the donors; **** P -value <.0001 calculated using paired t -test; *** P -value =.0005 calculated using Mann–Whitney test. C , Percent similarity of the repeated FMT recipient samples at those time points to the respective donor of the most recent FMT (PFT1: 1–2 weeks; PFT2: 3–28 weeks); * P -value <.05; ** P -value <.005 both calculated using Mann–Whitney test. Whiskers denote mean with the standard deviation. Abbreviation: BFT, before FMT.

Article Snippet: DNA was extracted and analyzed on a custom oligonucleotide-based DNA microarray containing 370 probes targeting 354 ABR genes (Lallemand Health Solutions, Montreal, Canada).

Techniques: Infection, Microarray, Transplantation Assay, MANN-WHITNEY, Standard Deviation